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Fabricating Multiphasic Angiogenic Scaffolds Using Amyloid/Roxadustat-Assisted High-Temperature Protein Printing.
Mohsen Akbarian1, Maryam Kianpour1, Lobat Tayebi1
1Marquette University School of Dentistry, Milwaukee, Wisconsin 53233, United States.
ACS Applied Materials & Interfaces
|July 2, 2024
Summary
New human serum albumin (HSA) scaffolds, both soft and hard, promote tissue repair by enhancing cell attachment, proliferation, and blood vessel formation. These advanced biomaterials offer a promising solution for complex tissue defect regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Repairing complex multiphasic tissue defects remains a significant clinical challenge.
- Existing scaffolds often lack the necessary properties for effective vascularization and cell integration.
- Developing advanced biomaterials is crucial for enhancing tissue regeneration outcomes.
Purpose of the Study:
- To design and fabricate novel soft and hard scaffolds using human serum albumin (HSA) fibrils.
- To evaluate the efficacy of these scaffolds in promoting cell attachment, proliferation, migration, and metabolism.
- To assess the angiogenic potential and suitability for multiphasic defect regeneration.
Main Methods:
- Fabrication of thermostable, printable hard polymers and injectable hydrogels using human serum albumin (HSA) fibrils and chitosan-conjugated arginine.
- In vitro assessment of scaffold interaction with human periodontal ligament fibroblasts (PDLF), human umbilical vein endothelial cells (HUVEC), and human osteoblasts.
- Ex ovo and in vitro experiments to evaluate vascularization, angiogenesis, and cell cross-talk in a coculture system with roxadustat.
Main Results:
- HSA-F based scaffolds significantly enhanced attachment, migration, proliferation, and metabolism of PDLF, HUVEC, and osteoblasts.
- The multiphasic scaffolds promoted vascularization and angiogenesis, increasing blood vessel density.
- Coculture of PDLF and HUVEC with roxadustat in the scaffolds demonstrated effective cell cross-talk and angiogenic response.
Conclusions:
- Novel HSA-F based soft and hard scaffolds effectively support cell attachment and proliferation, crucial for tissue regeneration.
- The developed scaffolds demonstrate significant potential for enhancing angiogenesis and vascularization in defect sites.
- This approach offers a promising strategy for the regeneration of complex multiphasic tissue defects by fostering a conducive environment for healing.
