JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Salivary oxidative stress biomarkers in periodontitis-free smokers: a cross sectional study

  • 0Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates - malrashdan@sharjah.ac.ae.
Minerva Dental and Oral Science +

|

July 2, 2024

|

July 2, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Salivary oxidative stress markers showed subtle changes between cigarette smokers, waterpipe smokers (WPS), and controls. However, smoking habits did not significantly influence these oxidative stress biomarkers in periodontitis-free individuals.

Area Of Science

  • Biochemistry
  • Oral Health
  • Toxicology

Background

  • Salivary oxidative stress is linked to local and systemic factors, including smoking.
  • Previous studies explored correlations between oxidative stress markers and smoking, but comparisons between different smoking methods are limited.

Purpose Of The Study

  • To investigate the impact of cigarette smoking versus waterpipe smoking (WPS) on salivary oxidative stress biomarkers.
  • To compare oxidative stress levels in periodontitis-free cigarette smokers, WPS users, and non-smokers.

Main Methods

  • Three groups were studied: controls (never smokers), cigarette smokers, and WPS users, all free from periodontitis and systemic conditions.
  • Saliva samples were analyzed for 8-hydroxy-2'-deoxyguanosine (8-OHdG), myeloperoxidase (MPO), malondialdehyde (MDA), total antioxidant capacity (TAC), and cortisol.
  • One-way ANOVA and hierarchical linear regression were used for statistical analysis.

Main Results

  • Waterpipe smoking (WPS) group had the highest 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels.
  • Cigarette smokers exhibited the highest malondialdehyde (MDA) and cortisol levels.
  • The WPS group showed the highest total antioxidant capacity (TAC), but no statistically significant differences were observed for any biomarker across groups.

Conclusions

  • Salivary oxidative stress does not appear to be significantly affected by cigarette or waterpipe smoking habits in individuals without periodontitis.
  • Subtle alterations in some oxidative stress biomarkers were noted, but lacked statistical significance.