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Updated: Jun 22, 2025

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry
Published on: September 10, 2018
Discovery and Quantitative Dissection of Cytokinesis Mechanisms Using Dictyostelium discoideum
Jennifer M K Nguyen1,2, Yinan Liu1,2, Ly Nguyen1
1Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Abstract:
The social amoeba Dictyostelium discoideum is a versatile model for understanding many different cellular processes involving cell motility including chemotaxis, phagocytosis, and cytokinesis. Cytokinesis, in particular, is a model cell-shaped change process in which a cell separates into two daughter cells. D. discoideum has been used extensively to identify players in cytokinesis and understand how they comprise the mechanosensory and biochemical pathways of cytokinesis. In this chapter, we describe how we use cDNA library complementation with D. discoideum to discover potential regulators of cytokinesis. Once identified, these regulators are further analyzed through live cell imaging, immunofluorescence imaging, fluorescence correlation and cross-correlation spectroscopy, micropipette aspiration, and fluorescence recovery after photobleaching. Collectively, these methods aid in detailing the mechanisms and signaling pathways that comprise cell division.
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