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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: Jun 19, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Graphene Multiplexed Sensor for Point-of-Need Viral Wastewater-Based Epidemiology.

Michael Geiwitz¹, Owen Rivers Page², Tio Marello¹

¹Department of Physics, Boston College, Chestnut Hill, Massachusetts 02467, United States.

ACS Applied Bio Materials

|July 2, 2024

Summary

A new biosensing platform offers low-cost, rapid detection of respiratory viruses like SARS-CoV-2 in wastewater. This wastewater-based epidemiology tool enables early outbreak detection, especially in resource-limited areas.

Keywords:

SARS-CoV-2 aptamer caffeine graphene field effect transistor (GFET)influenza respiratory syncytial virus

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Last Updated: Jun 19, 2026

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Published on: March 13, 2018

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Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Area of Science:

Environmental microbiology
Biosensor technology
Public health surveillance

Background:

Wastewater-based epidemiology (WBE) is crucial for tracking infectious diseases like SARS-CoV-2, RSV, and influenza.
Current WBE methods are often costly and slow, limiting their application in low-resource settings and small populations.
There is a growing need for accessible, rapid, and cost-effective biosensing platforms for local virus detection.

Purpose of the Study:

To develop an easy-to-use, cost-effective, multiplexed biosensing platform for detecting viral loads in wastewater.
To achieve a lower limit of detection compared to traditional methods like mass spectrometry.
To enable rapid, local monitoring of respiratory virus outbreaks.

Main Methods:

Developed a wafer-scale production method for biosensor chips with pre-attached aptamers.
Each chip contains 20 transistors configured to detect four analytes simultaneously.
Utilized aptamers for specific binding to target viral proteins and a normalization molecule.

Main Results:

The platform demonstrated a significantly lower limit of detection than mass spectrometry.
Successfully detected viral proteins from SARS-CoV-2, Respiratory Syncytial Virus (RSV), and Influenza A in wastewater samples.
Detected caffeine as a population normalization molecule, enabling accurate viral load quantification.

Conclusions:

The developed biosensing platform is a cost-effective and sensitive tool for wastewater-based epidemiology.
Its ease of use and multiplexing capabilities facilitate rapid, local detection of key respiratory viruses.
Future development into hand-held devices could revolutionize outbreak prevention in resource-limited settings.