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Updated: Jun 22, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Indopolycarbocyanine dyes as perspective analytical reagents for spectrophotometric determination of nitrite by
Andrii R Shabelko1, Nadiya A Derevyanko2, Alexander A Ishchenko2
1Taras Shevchenko National University of Kyiv 01601, Kyiv, Str. Volodymyrska, 64/13, Ukraine.
Abstract:
A spectrophotometric method for the quantitative determination of nitrite was developed, based on the radical nitration of indopolycarbocyanine dyes in the presence of 2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO). The rate of the reaction of the studied dyes with nitrite increases with the lengthening of the polymethine chain and the presence of hydrophilic sulfo groups in the side chain of the dye. TEMPO acts as a co-reagent, significantly accelerating the reaction rate and increasing the sensitivity of nitrite determination. The proposed reaction mechanism is supported by spectrophotometric and HPLC/MS studies. For Ind2 (tetramethine indocarbocyanine cationic dye), the limit of detection for nitrite is 0.50 µM within a linearity range of 1-13 µM. The developed method is sensitive, with a LOD 130 times lower than the maximum contaminant level (MCL) of nitrite in drinking water (65 μM), as specified by the WHO. The method is of low-toxicity and good selectivity, as the determination of nitrite is not significantly affected by the main components of water. The method was successfully applied for the analysis of nitrite in natural and bottled water.
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