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Peroxiredoxin-2 represses NRAS-mutated melanoma cells invasion by modulating EMT markers

Isabella Harumi Yonehara Noma1, Larissa Anastacio da Costa Carvalho1, Denisse Esther Mallaupoma Camarena1

  • 1Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 580, São Paulo, SP 05508-00, Brazil.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|July 2, 2024
PubMed

Insights

Researchers found that PRDX2 influences aggressive traits in NRAS-mutated melanoma. Lower PRDX2 levels promote cancer spread, suggesting PRDX2 as a potential biomarker and therapeutic target for this challenging melanoma subtype.

Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • NRAS mutations are common in aggressive melanoma, often leading to therapy resistance and poor outcomes.
  • Cellular plasticity contributes to the challenges in treating advanced and resistant melanoma.
  • Peroxiredoxins (PRDXs) are key regulators of cellular processes via hydrogen peroxide oxidation and redox signaling.

Purpose of the Study:

  • To investigate the role of PRDX2 in NRAS-mutated melanoma.
  • To explore PRDX2 as a potential modulator of epithelial-mesenchymal transition (EMT) and cellular plasticity.
  • To evaluate PRDX2 as a prognostic biomarker and therapeutic target in NRAS-mutated melanoma.

Main Methods:

  • PRDX2 knockdown experiments in human reconstructed skin models.
  • Treatment of PRDX2-deficient cells with a PRDX mimetic (gliotoxin).
  • Analysis of PRDX2 expression in a large primary melanoma patient cohort.

Main Results:

  • PRDX2 knockdown induced phenotypic changes associated with invasion in NRAS-mutated melanoma models.
  • Gliotoxin treatment reduced migration in PRDX2-deficient melanoma cells.
  • High PRDX2 expression correlated with favorable clinical outcomes in patients with primary melanoma.

Conclusions:

  • PRDX2 acts as a modulator of EMT markers in NRAS-mutated melanomas.
  • PRDX2 plays a role in regulating melanoma cell migration and invasion.
  • PRDX2 presents a promising biomarker and therapeutic target for NRAS-mutated melanoma.
Keywords:
Cutaneous malignant melanomaGliotoxin (PubChem CID: 6223)Peroxiredoxin 2Phenotype switching

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