Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

2.0K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
2.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

1.0K
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
1.0K
Microbial Biosensors01:17

Microbial Biosensors

58
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
58

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MSDF-Net: a cross-version lightweight detection framework based on deformable convolution and high-resolution feature enhancement for pine wilt disease.

Frontiers in plant science·2026
Same author

ESE-PWDNet: an efficient early-stage pine wilt disease detection network.

Frontiers in plant science·2026
Same author

Multilevel Proteome Analysis Reveals the Region-specific Components of the Human Milk Fat Globule Membrane in China.

Journal of agricultural and food chemistry·2026
Same author

A Phase II Trial of Perioperative Camrelizumab Plus Neoadjuvant Chemotherapy in Resectable Stage IIB-IIIB Lung Squamous Cell Carcinoma.

MedComm·2026
Same author

Construction and application of a microfluidic-droplet system for high-throughput screening of amylase-producing strains from jiuqu.

The Analyst·2026
Same author

The diagnosis and management of ectopic thyroid cancer: a systematic review.

Frontiers in endocrinology·2026
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Apr 8, 2026

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
16:19

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

11.7K

Real-Time pH Sensor in Bacterial Microenvironments Using Liquid Crystal Core-Shell Microspheres.

Yaoshuang Xie1, Yuxuan Li2, Haifeng Lin1

  • 1MOE Key Laboratory of Geriatric Nutrition and Health, Department of Bioengineering, Beijing Technology and Business University, Beijing 100048, China.

Analytical Chemistry
|July 3, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel liquid crystal (LC) microsphere biosensor for real-time, 3D monitoring of bacterial microenvironments. The biosensor accurately detects localized pH changes, revealing distinct bacterial acidifying or alkalizing effects.

More Related Videos

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
11:18

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Published on: January 7, 2019

8.5K
In vitro Monitoring of Extracellular pH in Real-Time
10:11

In vitro Monitoring of Extracellular pH in Real-Time

Published on: June 3, 2021

1.9K

Related Experiment Videos

Last Updated: Apr 8, 2026

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
16:19

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

11.7K
Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
11:18

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Published on: January 7, 2019

8.5K
In vitro Monitoring of Extracellular pH in Real-Time
10:11

In vitro Monitoring of Extracellular pH in Real-Time

Published on: June 3, 2021

1.9K

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Microbiology

Background:

  • The bacterial microenvironment significantly influences bacterial growth and behavior.
  • Localized monitoring of microenvironmental factors is crucial for understanding bacterial adaptation to stimuli.
  • Existing methods may lack the resolution or real-time capabilities for detailed microenvironment analysis.

Purpose of the Study:

  • To develop a novel liquid crystal (LC) biosensor in a microsphere configuration for real-time 3D monitoring of the bacterial microenvironment.
  • To demonstrate the biosensor's capability for localized pH monitoring within bacterial cultures.
  • To investigate the distinct pH changes induced by different bacterial species.

Main Methods:

  • Development of a core-shell LC-Gel microsphere biosensor incorporating a nematic liquid crystal (LCE7) doped with 4-pentylbiphenyl-4'-carboxylic acid (PBA).
  • Encapsulation of bacteria within the shell of the microsphere.
  • Utilizing polarized optical microscopy to observe LC configuration changes in response to localized pH variations.
  • Implementation within a microfluidic chip for controlled experiments.

Main Results:

  • The LC-Gel microsphere biosensor successfully achieved real-time, localized pH monitoring with a resolution of 0.1.
  • Protonation of PBA's carboxyl groups induced observable transitions in the LC nucleus (axial to bipolar) due to altered charge density and molecular orientation.
  • Diverse bacterial species exhibited distinct acidifying or alkalizing effects on the microenvironment, as detected by the biosensor.

Conclusions:

  • The developed LC-Gel microsphere biosensor offers a versatile, label-free tool for precise, localized pH monitoring in bacterial microenvironments.
  • This technology facilitates the investigation of chemical and mechanical stimuli effects on bacterial metabolism.
  • The findings open new avenues for studying complex bacterial community dynamics and responses.