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Updated: Jun 22, 2025

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
A novel fluorescent probe with a phosphofluorene molecular structure for selective detection of hydrogen sulfide in
Shuntao Zhang1, Xingyong Liu1, Xiangjun Chen1
1College of Chemical Engineering, Sichuan University of Science & Engineering Zigong 643000 China wangjuan2022@suse.edu.cn.
Abstract:
Hydrogen sulfide (H2S) gas plays a significant role in biological regulation. With advancements in technology, H2S has been discovered across diverse fields, necessitating a comprehensive understanding of its physiological functions through monitoring changes in H2S within complex environments and physiological processes. In this study, we designed a phosphofluorene-based conjugate probe PPF-CDNB with an asymmetric π-conjugated phosphine structure and utilized dinitrophenyl ether as the recognition site for H2S. PPF-CDNB exhibited exceptional resistance to interference and demonstrated stability over a broad pH range (3.0-10.0), making it suitable for various environmental conditions. Intracellular experiments revealed that PPF-CDNB effectively monitored both endogenous and exogenous levels of H2S.
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