Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.8K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
Oogenesis02:07

Oogenesis

63.6K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.6K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Yap1 Suppression By Zdhhc7 Is Associated With Ferroptosis Resistance And Poor Prognosis In Ovarian Clear Cell Carcinoma

YAP1 Suppression by ZDHHC7 Is Associated with Ferroptosis Resistance and Poor Prognosis in Ovarian Clear Cell Carcinoma

Yoko Furutake1, Ken Yamaguchi1, Koji Yamanoi1

  • 1Department of Gynecology and Obstetrics, Graduate School of Medicine and Faculty of Medicine, Kyoto University, Kyoto, Japan.

Molecular Cancer Therapeutics
|July 3, 2024

Related Experiment Videos

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

931
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

6.9K
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

10.8K

View abstract on PubMed

Summary
This summary is machine-generated.

Ovarian clear cell carcinoma (OCCC) cells resist ferroptosis, a cell death pathway. Targeting YAP1 activation, regulated by ZDHHC7, may overcome this resistance and treat OCCC.

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Ovarian clear cell carcinoma (OCCC) presents unique clinical features and poor prognosis, often linked to therapeutic resistance.
  • OCCC develops in an oxidative stress environment due to iron accumulation, suggesting inherent resistance mechanisms.
  • Ferroptosis, an iron-dependent cell death, is a potential therapeutic target, but its role in OCCC resistance is unclear.

Purpose of the Study:

  • To investigate OCCC's resistance to ferroptosis.
  • To elucidate the underlying molecular mechanisms of ferroptosis resistance in OCCC.
  • To explore therapeutic strategies targeting ferroptosis in OCCC.

Main Methods:

  • Comparative analysis of OCCC cells and ovarian high-grade serous carcinoma cells under oxidative stress.

Related Experiment Videos

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

931
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

6.9K
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

10.8K
  • Cell viability assays using ferroptosis inducers like erastin.
  • Samroc analysis to assess Hippo signaling pathway enrichment.
  • Investigation of the role of Yes-associated protein 1 (YAP1) and ZDHHC7 in ferroptosis.
  • Mouse xenograft models to evaluate therapeutic efficacy.

    • Main Results:

    • OCCC cells exhibit resistance to ferroptosis-inducing agents despite oxidative stress.
    • The Hippo signaling pathway, particularly YAP1, is implicated in OCCC ferroptosis resistance.
    • Low nuclear YAP1 expression correlates with poor OCCC prognosis.
    • YAP1 activation enhances ferroptosis in OCCC cells.
    • Suppression of ZDHHC7 promotes ferroptosis via YAP1 activation.

    Conclusions:

    • OCCC cells possess intrinsic resistance to ferroptosis, mediated by the Hippo signaling pathway and YAP1.
    • ZDHHC7 plays a role in regulating ferroptosis sensitivity in OCCC through YAP1.
    • Targeting ferroptosis resistance by modulating YAP1 activation presents a promising therapeutic avenue for OCCC.