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Updated: Jun 22, 2025

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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MicroRNA-138 promotes the progression of multiple myeloma through targeting paired PAX5

Xiao Yan1, Keting Wang2, Cong Shi3

  • 1Department of Haematology, The First Affiliated Hospital of Ningbo University, China; Ningbo Clinical Research Center for Hematologic malignancies, China.

Mutation Research
|July 3, 2024
PubMed
Abstract

Insights

MicroRNA-138 (miR-138) promotes multiple myeloma (MM) progression and drug resistance by targeting PAX5. Downregulating miR-138 may offer a new therapeutic strategy for MM patients.

Area of Science:

  • Hematology
  • Oncology
  • Molecular Biology

Background:

  • Multiple myeloma cancer stem cells (MMSC) drive drug resistance and relapse in multiple myeloma (MM).
  • MicroRNAs (miRNAs) are implicated in MM progression, but the role of miR-138 in MMSC remains unclear.

Purpose of the Study:

  • To investigate the mechanism and role of miR-138 in multiple myeloma.

Main Methods:

  • Collected bone marrow and peripheral blood samples.
  • Isolated MMSC using a Magnet-based Cancer Stem Cell Isolation Kit.
  • Quantified gene and protein expression via RT-qPCR and Western blot.
  • Assessed MMSC proliferation and apoptosis using MTT and flow cytometry.
  • Confirmed PAX5 as a direct miR-138 target using dual-luciferase reporter assays.

Main Results:

  • miR-138 expression was significantly upregulated in MM patients compared to controls.
  • miR-138 expression negatively correlated with PAX5 expression.
  • Downregulation of miR-138 inhibited MMSC proliferation and promoted apoptosis in vitro and in vivo.
  • miR-138 regulated PAX5, Bcl-2, Bax, and Caspase-3 expression.

Conclusions:

  • miR-138 acts as an oncomiR in MM, promoting MMSC proliferation and survival by targeting PAX5.
  • miR-138 represents a potential therapeutic target for multiple myeloma treatment.
Keywords:
ApoptosisMMSCMiR-138PAX5Proliferation

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