Anaerobic peptostreptococcal keratitis

Begin with an anaerobic digester, a sealed, oxygen-free reactor seeded with a synthetic sludge that mimics the settled solids from wastewater.The sludge contains mesophilic anaerobic microbes, a mixed microbial community.These microbes thrive at moderate temperatures and drive the digestion of the sludge’s organic matter.Continuously mix the sludge to sustain the bacterial activity and promote digestion.Later, add bioplastics, which are biodegradable polymers, into the sludge.During...

Source: Wagner, A. O.,et al., Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions. J. Vis. Exp. (2019)This video demonstrates the preparation and inoculation of anaerobic cultures using oxygen-free sludge under strictly controlled, deoxygenated...

Here, we propose three different methods of damaging the sensory fibres innervating the cornea. These methods facilitate the study of axon regeneration in mice. These three methods, which are adaptable to other animal models, are ideal for the study of corneal innervation physiology and...

Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile...

Most studies of herpetic corneal disease use a primary infection model. However, primary infection with HSV-1 does not typically lead to human disease. Here we describe a recurrent model of herpetic corneal disease, which more closely mimics human...

In this video, we demonstrate a procedure to visualize internalized Porphyromonas gingivalis, an anaerobic pathogenic bacterium, within human endothelial cells using confocal microscopy. The bacteria are stained with a fluorescent dye while the host endothelial cell actin cytoskeleton is labeled with a different fluorescent dye. The internalized Porphyromonas gingivalis inside endothelial cells are visualized using confocal...