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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

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Fbxo28 is essential for spindle migration and morphology during mouse oocyte meiosis I

Haoya Chang1, Chenyang Huang2, Siyu Cheng3

  • 1Department of Obstetrics and Gynecology, Tangdu Hospital, Air Force Medical University, Xi'an 710038, China; Department of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen, China.

International Journal of Biological Macromolecules
|July 3, 2024
PubMed

Insights

Fbxo28 is crucial for mouse oocyte asymmetric division by regulating spindle assembly and migration. Its depletion impairs spindle morphology and actin dynamics, impacting fertility.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Reproductive Biology

Background:

  • Asymmetric oocyte division is vital for fertility, relying on precise spindle migration and assembly.
  • Fbxo28, an SCF ubiquitin E3 ligase component, is oocyte-specific, but its meiotic functions remain unclear.

Purpose of the Study:

  • To investigate the role of Fbxo28 in spindle assembly and migration during mouse oocyte meiosis I.
  • To elucidate Fbxo28's contribution to asymmetric division and identify its interacting cellular components.

Main Methods:

  • Morpholino-mediated knockdown and mRNA rescue experiments in mouse oocytes.
  • Immunofluorescence staining, western blotting, and time-lapse confocal microscopy.
  • Chromosome spreading and analysis of actin cytoskeleton organization.

Main Results:

  • Fbxo28 localizes to chromosomes and acentriolar microtubule-organizing centers (aMTOCs).
  • Fbxo28 depletion causes spindle morphology and migration defects, failing asymmetric division without affecting polar body extrusion.
  • Absence of Fbxo28 disrupts actin assembly and reduces ARPC2/ARP3 expression, with defects rescued by Fbxo28-myc mRNA.

Conclusions:

  • Fbxo28 plays a critical role in regulating spindle morphology and actin-based spindle migration during mouse oocyte maturation.
  • Fbxo28 is essential for successful asymmetric division, impacting fertility through its control of cytoskeletal dynamics.
Keywords:
AneuploidyFbxo28Meiosis progressionMouse oocyteSpindle migration

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