Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of two CASK patient-derived human induced pluripotent stem cell lines to study CASK-related disorders.

Stem cell research·2026
Same author

Adaptive evolution of gene regulatory networks in mammalian neocortex.

Nature·2026
Same author

Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells.

The EMBO journal·2026
Same author

Patient-derived organoids and neurospheres recapitulate salient features of primary tumor heterogeneity.

Neuro-oncology advances·2026
Same author

Histone methyltransferase PRDM9 promotes survival of drug-tolerant persister cells in glioblastoma.

Nature communications·2025
Same author

Protocol to analyze changes in hippocampal neural stem cell quiescence from single-cell RNA sequencing data.

STAR protocols·2025

Related Experiment Video

Updated: Jun 22, 2025

Subtype-selective Electroporation of Cortical Interneurons
06:42

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

8.8K

Polycomb repressive complex 2 is critical for mouse cortical glutamatergic neuron development.

Laura Currey1, Benjamin Mitchell1, Majd Al-Khalily2

  • 1School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia.

Cerebral Cortex (New York, N.Y. : 1991)
|July 3, 2024
PubMed
Summary

Polycomb Repressive Complex 2 (PRC2) mutations impact mature brain structure and glutamatergic neuron identity. This epigenetic modifier

Keywords:
EedH3K27me3PRC2glutamatergic neuronshistone modification

More Related Videos

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
04:17

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

Published on: March 8, 2024

857
Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
12:01

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Published on: January 12, 2015

10.2K

Related Experiment Videos

Last Updated: Jun 22, 2025

Subtype-selective Electroporation of Cortical Interneurons
06:42

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

8.8K
Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
04:17

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

Published on: March 8, 2024

857
Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
12:01

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Published on: January 12, 2015

10.2K

Area of Science:

  • Neuroscience
  • Epigenetics
  • Developmental Biology

Background:

  • Polycomb Repressive Complex 2 (PRC2) is crucial for corticogenesis.
  • PRC2 mutations are linked to human neurodevelopmental disorders due to haploinsufficiency.
  • The role of PRC2 in the mature brain remains under-explored.

Purpose of the Study:

  • To investigate the function of PRC2 in mature cortical structure and function.
  • To determine the consequences of conditional Eed deletion in the developing mouse dorsal telencephalon.

Main Methods:

  • Conditional deletion of the PRC2 gene Eed in the developing mouse dorsal telencephalon.
  • Analysis of adult homozygous and heterozygous mice.
  • Single-nucleus transcriptomics to assess gene expression profiles.
  • Evaluation of neuronal morphology and connectivity.

Main Results:

  • Homozygous mice exhibited smaller forebrain structures and affected glutamatergic neurons with altered gene expression, morphology, and connectivity.
  • Despite anatomical and gene expression changes, homozygous mice performed well on cognitive tasks.
  • Heterozygous mice showed distinct neuronal gene dysregulation and morphological alterations compared to homozygotes, without clear anatomical or behavioral deficits.

Conclusions:

  • Alterations in PRC2 function significantly shape the mature brain.
  • PRC2 plays a dose-specific role in maintaining glutamatergic neuron identity.
  • Conditional Eed deletion reveals distinct homozygous and heterozygous phenotypes impacting neuronal development and function.