Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

177
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
177
Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

2.0K
Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
2.0K
The Electron Transport Chain01:30

The Electron Transport Chain

16.5K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
16.5K
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

134
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
134
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

14.4K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
14.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chain-Mobility-Enabled 1D Lanthanide Coordination Polymer Glassy Scintillators for Underwater X-Ray Videography.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Mainland diversification and recent island lineages in the reduviid genus Tapirocoris: an integrative taxonomic framework with four new species.

Insect science·2026
Same author

Fundamental study on the germination, revival of urease activity, and bio-deposition behaviors of Bacillus sphaericus spores for microbial self-healing concrete.

Environmental research·2026
Same author

EC-Dock: A Fast Equivariant Consistency Model for Molecular Docking and Virtual Screening.

Journal of chemical information and modeling·2026
Same author

Stimuli-Responsive Triplet Emission and X-Ray Scintillation via Reversible Structural Switching in Pyromellitic Diimide Cocrystals.

Angewandte Chemie (International ed. in English)·2026
Same author

Quercetin Ameliorates Renal Fibrosis via SIRT5 in Diabetic Nephropathy.

Iranian journal of kidney diseases·2026
Same journal

Daily briefing: How cooperation built the world.

Nature·2026
Same journal

Deep-sea oddities and boatloads of other new species - June's best science images.

Nature·2026
Same journal

From cloning to gene-editing: the enduring legacy of Dolly the sheep.

Nature·2026
Same journal

Time to give hydration breaks the red card? What science says about keeping cool.

Nature·2026
Same journal

Universities are relying on AI-detection software to catch cheating. How well do the programs work?

Nature·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2025

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

8.6K

Inhibition of M. tuberculosis and human ATP synthase by BDQ and TBAJ-587.

Yuying Zhang1, Yuezheng Lai1, Shan Zhou1

  • 1State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, China.

Nature
|July 3, 2024
PubMed
Summary
This summary is machine-generated.

Bedaquiline and TBAJ-587 inhibit tuberculosis growth by targeting ATP synthase. Cryo-EM structures reveal similar binding sites in M. tuberculosis and human ATP synthase, aiding new drug design.

More Related Videos

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
12:17

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

Published on: May 8, 2017

11.8K
Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments
07:50

Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments

Published on: October 25, 2024

1.6K

Related Experiment Videos

Last Updated: Jun 22, 2025

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

8.6K
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
12:17

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

Published on: May 8, 2017

11.8K
Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments
07:50

Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments

Published on: October 25, 2024

1.6K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Drug Discovery

Background:

  • Bedaquiline (BDQ) is a crucial anti-tuberculosis drug targeting Mycobacterium tuberculosis ATP synthase.
  • BDQ also inhibits human ATP synthase, raising safety concerns.
  • The precise interaction mechanisms of BDQ and its analogues with ATP synthases remain incompletely understood.

Purpose of the Study:

  • To elucidate the structural basis of Bedaquiline (BDQ) and TBAJ-587 binding to Mycobacterium tuberculosis ATP synthase.
  • To determine the structure of human ATP synthase in complex with BDQ.
  • To compare the binding modes of BDQ in M. tuberculosis and human ATP synthases for rational drug design.

Main Methods:

  • Cryogenic electron microscopy (cryo-EM) was employed to determine high-resolution structures.
  • Structures were obtained for M. tuberculosis ATP synthase with and without BDQ and TBAJ-587.
  • The structure of human ATP synthase complexed with BDQ was also determined.

Main Results:

  • Cryo-EM structures reveal that BDQ and TBAJ-587 bind to M. tuberculosis ATP synthase at multiple sites, including subunit a and the c-ring.
  • Both compounds exhibit similar modes of action against M. tuberculosis ATP synthase.
  • The binding site of BDQ in human ATP synthase is structurally similar to a key site in M. tuberculosis ATP synthase, with extensive interactions observed.

Conclusions:

  • BDQ and TBAJ-587 share similar binding interactions within M. tuberculosis ATP synthase.
  • The structural similarity in BDQ binding between M. tuberculosis and human ATP synthases provides insights into potential off-target effects.
  • These findings facilitate the rational design of novel diarylquinoline derivatives with improved anti-tuberculosis efficacy and safety profiles.