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The correlation between the MYBL2/CDCA8 signaling pathway of malignant melanoma

Chen Wu1, Jiahui Jiang1, Chao Ci1

  • 1Department of Dermatology, The First Affiliated Hospital of Wannan Medical College, No. 2 Zheshan West Road, Wuhu, Anhui, 241001, China.

Heliyon
|July 4, 2024
PubMed
Abstract

Insights

MYB proto-oncogene like 2 (MYBL2) promotes malignant melanoma cell migration and invasion by regulating Cell division cycle associated 8 (CDCA8) expression. This study reveals MYBL2 as a potential therapeutic target for melanoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Cutaneous malignant melanoma is an aggressive skin cancer with significant mortality.
  • Understanding the molecular mechanisms driving melanoma progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of MYB proto-oncogene like 2 (MYBL2) in regulating Cell division cycle associated 8 (CDCA8) expression.
  • To determine the impact of MYBL2-mediated CDCA8 regulation on the biological activity of cutaneous malignant melanoma cells.

Main Methods:

  • Utilized A375 melanoma cells with MYBL2 and CDCA8 overexpression and knockdown.
  • Assessed cell migration, invasion, and proliferation using in vitro assays.
  • Quantified apoptosis via flow cytometry and evaluated in vivo tumorigenic potential in nude mice.

Main Results:

  • MYBL2 and CDCA8 expression was elevated in melanoma cells compared to normal cells.
  • MYBL2 downregulation reduced melanoma cell migration and invasion, while overexpression enhanced these processes.
  • A positive correlation was observed between MYBL2 and CDCA8 expression.
  • In vivo studies showed MYBL2 knockdown significantly reduced tumor volume and CDCA8 expression.

Conclusions:

  • MYBL2 promotes migration, invasion, and proliferation in cutaneous malignant melanoma cells.
  • This promotion is achieved through the targeted regulation of CDCA8 expression.
  • MYBL2 represents a potential therapeutic target for melanoma treatment.
Keywords:
Cell division cycle associated 8MYB proto-oncogene like 2Malignant melanoma

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