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Updated: Jun 22, 2025

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
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Knockdown of hsa_circ_0102231 Impedes the Progression of Liver Cancer through the miR-873-SOX4 Axis

Jingyu Qian1, Banghong Jiang2, Zhongqiang Qin1

  • 1Department of Interventional Radiology, The First Affiliated Hospital of Bengbu Medical University, Anhui, Bengbu, 233004, People's Republic of China.

Current Gene Therapy
|July 4, 2024
PubMed
Abstract

Insights

Circular RNA 0102231 (hsa_circ_0102231) promotes liver cancer progression by regulating the miR-873/SOX4 axis. Inhibiting hsa_circ_0102231 impedes hepatocellular carcinoma cell proliferation and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) presents significant challenges due to high recurrence and heterogeneity.
  • Understanding molecular mechanisms driving HCC progression is crucial for effective treatment strategies.

Purpose of the Study:

  • To investigate the role of circular RNA 0102231 (hsa_circ_0102231) in hepatocellular carcinoma (HCC) progression.
  • To elucidate the molecular pathway regulated by hsa_circ_0102231 in liver cancer.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) to measure hsa_circ_0102231 expression.
  • Bioinformatics, dual-luciferase reporter, and RNA pull-down assays to identify downstream targets.
  • Cell proliferation (colony formation, CCK8) and migration (Transwell) assays, and xenograft models to assess functional roles.

Main Results:

  • Hsa_circ_0102231 expression was significantly elevated in HCC cell lines (HepG2, Huh-7).
  • Knockdown of hsa_circ_0102231 suppressed HCC cell proliferation and migration in vitro and in vivo.
  • Hsa_circ_0102231 was found to target miR-873 and SOX4, with miR-873 inhibition or SOX4 overexpression rescuing proliferation and migration defects.

Conclusions:

  • Hsa_circ_0102231 knockdown inhibits liver cancer progression by modulating the miR-873/SOX4 axis.
  • Hsa_circ_0102231 warrants further investigation as a potential therapeutic target for HCC.
Keywords:
Liver cancerSOX4cancer progressioncell migration.hsa_circ_0102231miR-873

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