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Proteolytic activity of largomycin.

A Zaheer, S Zaheer, R Montgomery

    Biochimica Et Biophysica Acta
    |September 6, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Largomycin, an antibiotic and antitumor protein, exhibits specific proteolytic activity against certain peptides and cell membrane proteins. This activity, linked to its biological function, resides in its apoprotein component.

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    Area of Science:

    • Microbiology and Biochemistry
    • Protein Chemistry
    • Enzymology

    Background:

    • Largomycin, an antibiotic and antitumor protein from Streptomyces pluricolorescens, possesses uncharacterized biological activities.
    • Understanding the enzymatic properties of largomycin is crucial for elucidating its mechanism of action.

    Purpose of the Study:

    • To investigate the specific proteolytic activity of purified largomycin.
    • To determine the relationship between largomycin's biological and proteolytic activities.
    • To identify the component of largomycin responsible for proteolytic activity.

    Main Methods:

    • Purification of largomycin from Streptomyces pluricolorescens culture broth.
    • Assay of proteolytic activity using various peptide substrates and KB cell plasma membrane proteins.

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  • Comparison of temperature-dependent profiles for biological and proteolytic activities.
  • Characterization of the apoprotein's proteolytic activity.

    • Main Results:

    • Pure largomycin demonstrated specific proteolytic activity, degrading angiotensin II, bradykinin, dipeptides, and KB cell plasma membrane proteins.
    • Largomycin did not degrade common aminopeptidase, endopeptidase, or carboxypeptidase substrates.
    • Biological and proteolytic activities exhibited similar temperature dependencies, suggesting a single protein is responsible.
    • The apoprotein of largomycin retained proteolytic activity despite lacking antibiotic properties.

    Conclusions:

    • Largomycin possesses a unique proteolytic activity distinct from common peptidases.
    • The proteolytic activity is intrinsically linked to largomycin's biological functions (antibiotic and antitumor).
    • The apoprotein of largomycin is the functional entity responsible for its proteolytic and biological activities.