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Updated: Jun 22, 2025

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
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Valosin-Containing Protein (VCP)/p97 Oligomerization.

Guimei Yu1, Yunpeng Bai2, Zhong-Yin Zhang3

  • 1School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, China.

Sub-Cellular Biochemistry
|July 4, 2024
PubMed
Summary
This summary is machine-generated.

Valosin-containing protein (VCP), crucial for cellular homeostasis, exists in hexameric and dodecameric states. Understanding its oligomerization and regulation offers therapeutic targets for neurodegenerative diseases and cancers.

Keywords:
ALSCancerDodecamerHexamerIBMPFDNucleotide bindingOligomerizationUbiquitinationVCP/p97

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Biology

Background:

  • Valosin-containing protein (VCP/p97) is an AAA+ ATPase vital for cellular homeostasis.
  • VCP participates in protein degradation via the ubiquitin-proteasome system (UPS) and autophagy.
  • Mutations in VCP cause degenerative disorders like IBMPFD, ALS, and cancers, highlighting its therapeutic potential.

Purpose of the Study:

  • To summarize current knowledge on VCP oligomerization and regulation.
  • To explore the implications of VCP's oligomeric states in cellular function and disease.
  • To discuss VCP as a therapeutic target for neurodegeneration and cancer.

Main Methods:

  • Review of recent studies on VCP oligomerization.
  • Analysis of VCP's functional mechanisms involving cofactors.
  • Investigation of VCP's role in UPS and autophagy pathways.

Main Results:

  • VCP functions predominantly as a hexamer, extracting ubiquitinated substrates for degradation.
  • A novel dodecameric VCP state has been identified.
  • VCP oligomeric states are regulated by D2 domain nucleotide occupancy.

Conclusions:

  • VCP oligomerization is a key regulatory mechanism controlling its cellular functions.
  • Understanding VCP's diverse states and regulation is crucial for developing therapies.
  • Targeting VCP offers promise for treating VCP-associated neurodegenerative diseases and cancers.