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Preparation of poly(vinyl alcohol) nanofibers containing disulfiram-copper complex by electrospinning: a potential delivery system against melanoma

  • 0Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt. gelfawal@gmail.com.
Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences +

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July 4, 2024

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July 4, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study successfully encapsulated the disulfiram-copper complex into polyvinyl alcohol nanofibers. This novel DS-Cu@PVA scaffold enhances melanoma treatment by increasing drug bioavailability and reducing toxicity.

Area Of Science

  • Biomaterials Science
  • Nanotechnology
  • Cancer Therapeutics

Background

  • Melanoma presents a significant health challenge requiring effective treatments.
  • Disulfiram-copper complex (DS-Cu) shows promise as an anticancer agent.

Purpose Of The Study

  • To encapsulate the DS-Cu complex into a polyvinyl alcohol (PVA) nanofiber scaffold (DS-Cu@PVA).
  • To enhance bioavailability and reduce toxicity of DS-Cu for melanoma treatment.

Main Methods

  • Fabrication of the nanofiber scaffold using electrospinning.
  • Characterization via ART-Fourier transform infrared spectroscopy (ART-FTIR), scanning electron microscopy (SEM), and energy dispersive X-ray analysis (EDX).
  • Assessment of cellular cytotoxicity, flow cytometry, and caspase 3 activity.

Main Results

  • Successful encapsulation of DS-Cu into PVA nanofibers confirmed by characterization techniques.
  • SEM showed consistent nanofiber diameter; ATR-FTIR indicated no significant alteration of PVA peaks.
  • DS-Cu@PVA exhibited superior biocompatibility with normal skin cells and enhanced melanoma cell apoptosis via increased reactive oxygen species and caspase 3 activity.

Conclusions

  • The DS-Cu@PVA nanofiber scaffold demonstrates enhanced biocompatibility and potent cytotoxic effects on melanoma cells.
  • Targeted delivery of DS-Cu via PVA nanofibers offers a promising strategy for melanoma therapy.

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