Evaluation of Boric Acid Treatment on microRNA-127-5p and Metastasis Genes Orchestration of Breast Cancer Stem Cells
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View abstract on PubMed
Summary
This summary is machine-generated.Boric acid (BA) was investigated for its effects on breast cancer stem cells (BC-SCs) and miR-127-5p expression. BA showed anti-cancer properties by inhibiting BC-SC viability and upregulating miR-127-5p, but did not enhance metastasis.
Area Of Science
- Oncology
- Molecular Biology
- Biochemistry
Background
- MicroRNA (miRNA) and cancer stem cell (CSC) coregulation is crucial in carcinogenesis.
- miR-127-5p is frequently downregulated in breast cancer.
- Boric acid (BA) possesses unstudied anti-cancer properties.
Purpose Of The Study
- To investigate the effect of boric acid (BA) on miR-127-5p expression.
- To assess the impact of BA on genes associated with breast cancer stem cell (BC-SC) metastasis.
- To evaluate the anti-cancer potential of BA in BC-SCs.
Main Methods
- BC-SCs were isolated from MCF-7 cells using immunomagnetic separation.
- Cell viability and IC50 were determined using MTT assays.
- Gene and miRNA expression levels were analyzed via RT-qPCR.
Main Results
- Boric acid demonstrated dose- and time-dependent inhibition of BC-SC viability.
- BA treatment led to the upregulation of miR-127-5p, ZEB1, CDH1, ITGB1, ITGA5, LAMA5, and SNAIL.
- Expression of COL1A1 and VIM was downregulated by BA treatment.
Conclusions
- Boric acid can induce miR-127-5p expression in breast cancer stem cells.
- BA exhibits anti-cancer effects by inhibiting cell viability and modulating metastatic gene expression.
- Boric acid does not appear to enhance the metastatic potential of breast cancer stem cells.
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