F11R RNA trinucleotide over-edited by ADAR in gastric and colorectal cancers: Cross-cohort validation, gene expression regulation, and diagnostic significance
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View abstract on PubMed
Summary
This summary is machine-generated.A novel RNA editing event in the F11 receptor gene, mediated by adenosine deaminases acting on RNA (ADAR), is linked to gastric and colorectal cancers. This editing may serve as a new diagnostic biomarker for these diseases.
Area Of Science
- Molecular Biology
- Oncology
- Genetics
Background
- The F11 receptor (F11R) gene, encoding junctional adhesion molecule A, is implicated in gastric cancer (GC) and colorectal cancer (CRC).
- Adenosine-to-inosine (A-to-I) RNA editing, mediated by adenosine deaminases acting on RNA (ADARs), is a newly identified regulatory mechanism potentially affecting F11R.
Purpose Of The Study
- To investigate the role and regulation of F11R RNA editing in GC and CRC.
- To explore the clinical significance of ADAR-mediated F11R editing as a diagnostic biomarker.
Main Methods
- RNA sequencing (RNA-Seq) and experimental validation across multiple GC and CRC cohorts.
- Cell-based assays (overexpression, knockdown), Sanger sequencing, and crosslinking immunoprecipitation sequencing (CLIP-seq).
- Luciferase reporter assays to assess gene expression regulation.
Main Results
- An over-edited AAA trinucleotide in the F11R 3'-untranslated region (3'-UTR) was identified, correlated with elevated ADAR expression in GC and CRC.
- ADAR-mediated editing of the F11R 3'-UTR trinucleotide was confirmed, potentially disrupting an RBM45 binding site and regulating F11R expression.
- The F11R trinucleotide editing demonstrated promising predictive performance for GC and CRC diagnosis.
Conclusions
- ADAR-mediated editing of an F11R trinucleotide in the 3'-UTR has significant biological and clinical implications in GC and CRC.
- This specific RNA editing event represents a potential novel diagnostic biomarker for gastric and colorectal cancers.
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