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Fish decay-accelerating factor (DAF) regulates intestinal complement pathway and immune response to bacterial
Xia Zhang1, Yuhan Zhang2, Ting Wu2
1Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Department of Biological and Chemical Engineering, Changsha University, Changsha, 410022, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Department of Life Sciences, Hunan Normal University, Changsha, 410081, China.
Fish & Shellfish Immunology
|July 4, 2024
Summary
Decay-accelerating factor (DAF) in grass carp negatively regulates the intestinal immune response to bacterial infections. Enhancing DAF function reduced inflammation and modulated complement pathways, offering insights into fish immunity.
Area of Science:
- Immunology
- Fish Biology
- Complement System
Background:
- Decay-accelerating factor (DAF) is crucial for mammalian immune homeostasis.
- The immune role of DAF in bony fish, particularly in the intestine, remains under-investigated.
- Understanding DAF function in fish is vital for aquaculture and disease management.
Purpose of the Study:
- To characterize CiDAF from Ctenopharyngodon idella (grass carp).
- To investigate the role of CiDAF in intestinal immunity following bacterial infection.
- To explore CiDAF's potential as a therapeutic target for intestinal inflammation.
Main Methods:
- Characterization of CiDAF gene and protein structure.
- Gene expression analysis in various tissues and after pathogen challenge.
- In vivo RNA interference (RNAi) to assess CiDAF knockdown effects.
- In vitro experiments using a CiDAF agonist (progesterone) to treat inflammation.
Main Results:
- CiDAF exhibits conserved CCP domains and broad tissue expression, with high levels in spleen and kidney.
- CiDAF expression significantly responds to bacterial pathogens and PAMPs (LPS, MDP).
- CiDAF knockdown upregulated complement genes and inflammatory cytokines; CiDAF agonist alleviated intestinal inflammation.
Conclusions:
- CiDAF functions as a negative regulator of the intestinal complement pathway in grass carp.
- CiDAF plays a significant role in the immune response to bacterial challenges in fish.
- Modulating CiDAF activity presents a potential strategy for managing intestinal inflammation in grass carp.
Keywords:
Ctenopharyngodon idellaDecay-accelerating factorIntestinal immunityLipopolysaccharideMuramyl dipeptideMore Related Videos
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