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Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay
Published on: April 28, 2014
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Coptis rhizome extract influence on Streptococcus pneumoniae through autolysin activation
Eon-Bee Lee1,2, Kyubae Lee3
1Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea.
AMB Express
|July 4, 2024
Summary
Coptis rhizome extract CRET70 shows potent antibacterial activity against Streptococcus pneumoniae, including resistant strains. This traditional remedy effectively inhibits bacterial growth and biofilm formation, offering a promising natural alternative in the fight against antibiotic resistance.
Area of Science:
- Pharmacology
- Microbiology
- Ethnobotany
Background:
- Streptococcus pneumoniae causes significant respiratory infections.
- Antibiotic resistance in S. pneumoniae necessitates novel therapeutic strategies.
- Coptis rhizome is traditionally used for respiratory ailments, but its efficacy against S. pneumoniae lacks empirical validation.
Purpose of the Study:
- To investigate the antibacterial properties of Coptis rhizome extracts against S. pneumoniae.
- To evaluate the potential of Coptis rhizome in combating antibiotic resistance.
- To explore the mechanism of action, including effects on bacterial autolysis and biofilm formation.
Main Methods:
- Preparation of four Coptis rhizome extracts (CRET30, CRET50, CRET70, CRDW) using ethanol and distilled water.
- Assessment of antibacterial activity via Minimum Inhibitory Concentration (MIC), disk diffusion, and time-kill assays against standard and resistant S. pneumoniae strains.
- Evaluation of biofilm inhibition and monitoring of the lytA gene expression related to autolysis.
Main Results:
- The CRET70 extract exhibited potent antibacterial activity with an MIC of 0.125 µg/mL against both S. pneumoniae strains.
- CRET70 demonstrated significant inhibition zones (22.17 mm and 17.20 mm) and a 2-log decrease in bacterial counts.
- The extract effectively inhibited 77.40% of biofilm formation and induced significant overexpression of the lytA gene.
Conclusions:
- Coptis rhizome extract CRET70 possesses remarkable antibacterial efficacy against S. pneumoniae.
- The findings suggest Coptis rhizome as a potential natural therapeutic agent against S. pneumoniae infections, particularly in the context of rising antibiotic resistance.
- The lytA gene overexpression indicates autolysis as a potential mechanism contributing to the antibacterial effects of Coptis rhizome.
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