A solid state electrolyte based enzymatic acetone sensor

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Acetone detection is crucial for medical diagnostics, particularly for monitoring metabolic states.
• Existing acetone sensors face challenges in sensitivity, selectivity, and real-world applicability.
• Solid-state electrolyte-based enzymatic sensors offer a promising platform for improved acetone detection.

Purpose of the Study:

• To develop and optimize a novel solid-state electrolyte-based enzymatic sensor for acetone detection.
• To evaluate the performance of various surface modifications for enhanced sensing capabilities.
• To assess the sensor's suitability for breath acetone analysis.

Main Methods:

• Utilized disposable screen-printed electrodes with platinum and silver components.
• Employed cyclic voltammetry and amperometry for acetone concentration measurements.
• Investigated four distinct surface modifications using Nafion (N) and enzyme (E) layers (N+E, N+E+N, N+N+E, N+N+E+N).

Main Results:

• The sensor demonstrated high sensitivity and linearity (R² 0.92–0.98) for acetone in liquid and vapor phases (1 µM–25 mM).
• Achieved a rapid response time of 30–50 seconds and a low limit of detection of 0.03 µM.
• The four-layer modification (N+N+E+N) yielded optimal sensor performance.

Conclusions:

• The developed solid-state electrolyte-based enzymatic sensor is effective for acetone detection.
• The N+N+E+N surface modification significantly enhances sensor linearity, sensitivity, and detection limit.
• This optimized sensor shows potential for non-invasive breath acetone monitoring.