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Updated: Jun 22, 2025
A Preclinical Controlled Cortical Impact Model for Traumatic Hemorrhage Contusion and Neuroinflammation
Published on: June 10, 2020
CD300LF+ microglia impede the neuroinflammation following traumatic brain injury by inhibiting STING pathway
Zhichao Lu1,2,3,4, Zongheng Liu5, Chenxing Wang1,2,3,4
1Department of Neurosurgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, Jiangsu, China.
Introduction:
The diversity in microglial phenotypes and functions following traumatic brain injury (TBI) is poorly characterized. The aim of this study was to explore precise targets for improving the prognosis of TBI patients from a microglial perspective.
Objectives:
To assess whether the prognosis of TBI can be improved by modulating microglia function.
Results:
In CD300LF-deficient mice, we observed an increase in glial cell proliferation, more extensive neuronal loss, and worsened neurological function post-TBI. Transcriptomic comparisons between CD300LF-positive and CD300LF-negative microglia illuminated that the neuroprotective role of CD300LF is principally mediated by the inhibition of the STING signaling pathway. In addition, this protective effect can be augmented using the STING pathway inhibitor C-176.
Conclusions:
Our research indicates that CD300LF reduces neuroinflammation and promotes neurological recovery after TBI, and that microglia are integral to the protective effects of CD300LF in this context. In summary, our findings highlight CD300LF as a critical molecular regulator modulating the adverse actions of microglia following acute brain injury and propose a novel therapeutic approach to enhance outcomes for patients with TBI.
Insights
CD300LF protein protects the brain after traumatic brain injury (TBI) by reducing neuroinflammation. Modulating microglia function via CD300LF or STING inhibitors may improve TBI patient outcomes.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Microglial phenotypes and functions after traumatic brain injury (TBI) are not well understood.
- Identifying targets to improve TBI patient prognosis is crucial.
Purpose of the Study:
- To investigate if modulating microglia function can improve TBI prognosis.
- To explore the role of CD300LF in TBI.
Main Methods:
- Analysis of CD300LF-deficient mice post-TBI.
- Transcriptomic comparison of CD300LF-positive and -negative microglia.
- Assessment of STING signaling pathway inhibition.
Main Results:
- CD300LF deficiency worsened neurological function and neuronal loss post-TBI.
- CD300LF inhibits the STING signaling pathway, conferring neuroprotection.
- The STING inhibitor C-176 augmented the protective effects of CD300LF.
Conclusions:
- CD300LF reduces neuroinflammation and promotes recovery after TBI.
- Microglia mediate the protective effects of CD300LF.
- CD300LF is a potential therapeutic target for TBI.
