Genome sequence data of Saccharomyces cerevisiae CBS 493.94

Area of Science:

• Microbiology
• Genomics
• Yeast research

Background:

• Saccharomyces cerevisiae CBS 493.94 is a well-established yeast strain with historical significance, originating from a British brewery in 1958.
• This strain is widely utilized as a feed additive, contributing to animal health improvement.
• Comprehensive genomic data and strain identification are crucial for ensuring its safety and efficacy.

Purpose of the Study:

• To perform whole genome sequencing (WGS) on Saccharomyces cerevisiae CBS 493.94.
• To unambiguously identify the strain using multiple genetic markers.
• To assess the safety of the strain by screening for potential genes of concern, such as those related to antimycotic resistance.

Main Methods:

• Whole genome sequencing was conducted using both long-read (PacBio Sequel) and short-read (Illumina NovaSeq 6000) technologies.
• Strain identification was performed using 18S rRNA sequencing, Average Nucleotide Identity (ANI) dendrogram analysis, and Core Genome single nucleotide polymorphism (SNP) Tree construction.
• Sequence data was deposited in NCBI (PRJNA1044661) and the hybrid assembly was made publicly available via Zenodo and NCBI.

Main Results:

• The genetic analyses confirmed that the isolate belongs to the Saccharomyces cerevisiae species.
• No genes associated with antimycotic resistance were detected in the genome.
• Specific analysis of the FKS1 gene revealed no concerning amino acid variants.

Conclusions:

• The whole genome sequencing provides a definitive identification of Saccharomyces cerevisiae CBS 493.94.
• The absence of detected genes of concern supports the strain's safety profile for its intended use as a feed additive.
• This genomic data enhances the understanding and reliable application of this important yeast strain in animal health.