[Progress in enrichment methods for protein N-phosphorylation]

Bo Jiang ¹, Bo Gao ², Shu-Xian Wei ²

⁰State Key Laboratory of Medical Proteomics, National Chromatographic R.& A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Se Pu = Chinese Journal of Chromatography +

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July 5, 2024

View abstract on PubMed

Summary

Identifying protein N-phosphorylation is crucial for understanding its role in diseases. This review details N-phosphorylation enrichment methods, addressing limitations and future directions for large-scale identification and functional studies.

Area Of Science

Biochemistry and Molecular Biology
Proteomics
Post-Translational Modifications

Background

Protein phosphorylation, a key post-translational modification, regulates vital cellular processes and is implicated in diseases like cancer, neurodegeneration, and diabetes.
While O-phosphorylation is well-studied, N-phosphorylation (on histidine, arginine, lysine) is gaining attention for its role in signal transduction and metabolism, despite challenges in its study.
Current O-phosphorylation enrichment methods are unsuitable for N-phosphorylation due to the instability of the P-N bond under acidic conditions.

Purpose Of The Study

To address the urgent need for efficient enrichment methods for large-scale N-phosphorylation identification.
To review and analyze protein N-phosphorylation enrichment methods developed over the past two decades, detailing their advantages and disadvantages.
To promote further functional research on N-phosphorylation by summarizing current enrichment strategies and future directions.

Main Methods

Review of existing literature on protein N-phosphorylation enrichment techniques.
Detailed description of both antibody-based and non-antibody-dependent enrichment methods.
Analysis of the structural characteristics and biological functions of N-phosphorylation.

Main Results

N-phosphorylation is crucial in biological processes but is hindered by its instability and lack of suitable enrichment methods.
Antibody-based methods are currently limited to histidine phosphorylation due to molecular structure stability.
Non-antibody-dependent methods are also discussed, highlighting the need for new enrichment ligands.

Conclusions

The development of efficient and stable N-phosphorylation enrichment methods is critical for advancing research in this area.
Future research should focus on developing novel enrichment ligands to overcome current limitations and expand to other non-canonical phosphorylation targets.
This review provides a comprehensive overview of N-phosphorylation enrichment methods, guiding future research directions.

Keywords:

biological function enrichment method protein review