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Related Concept Videos

Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...

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Related Experiment Video

Updated: Jun 10, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
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[Progress in enrichment methods for protein N-phosphorylation].

Bo Jiang1, Bo Gao2, Shu-Xian Wei2

  • 1State Key Laboratory of Medical Proteomics, National Chromatographic R.& A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Se Pu = Chinese Journal of Chromatography
|July 5, 2024
PubMed
Summary
This summary is machine-generated.

Identifying protein N-phosphorylation is crucial for understanding its role in diseases. This review details N-phosphorylation enrichment methods, addressing limitations and future directions for large-scale identification and functional studies.

Keywords:
biological functionenrichment methodproteinreview

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Area of Science:

  • Biochemistry and Molecular Biology
  • Proteomics
  • Post-Translational Modifications

Background:

  • Protein phosphorylation, a key post-translational modification, regulates vital cellular processes and is implicated in diseases like cancer, neurodegeneration, and diabetes.
  • While O-phosphorylation is well-studied, N-phosphorylation (on histidine, arginine, lysine) is gaining attention for its role in signal transduction and metabolism, despite challenges in its study.
  • Current O-phosphorylation enrichment methods are unsuitable for N-phosphorylation due to the instability of the P-N bond under acidic conditions.

Purpose of the Study:

  • To address the urgent need for efficient enrichment methods for large-scale N-phosphorylation identification.
  • To review and analyze protein N-phosphorylation enrichment methods developed over the past two decades, detailing their advantages and disadvantages.
  • To promote further functional research on N-phosphorylation by summarizing current enrichment strategies and future directions.

Main Methods:

  • Review of existing literature on protein N-phosphorylation enrichment techniques.
  • Detailed description of both antibody-based and non-antibody-dependent enrichment methods.
  • Analysis of the structural characteristics and biological functions of N-phosphorylation.

Main Results:

  • N-phosphorylation is crucial in biological processes but is hindered by its instability and lack of suitable enrichment methods.
  • Antibody-based methods are currently limited to histidine phosphorylation due to molecular structure stability.
  • Non-antibody-dependent methods are also discussed, highlighting the need for new enrichment ligands.

Conclusions:

  • The development of efficient and stable N-phosphorylation enrichment methods is critical for advancing research in this area.
  • Future research should focus on developing novel enrichment ligands to overcome current limitations and expand to other non-canonical phosphorylation targets.
  • This review provides a comprehensive overview of N-phosphorylation enrichment methods, guiding future research directions.