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Published on: October 8, 2013
Mobile-CRISPRi protocol optimization for Vibrionaceae
Logan J Geyman1, Madeline P Tanner1, Natalia Rosario-Meléndez2,3
1Department of Biology, Indiana University , Bloomington, Indiana, USA.
Abstract:
Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromosome via Tn7 transposition. Here, we optimized methods specific for applying Mobile-CRISPRi in multiple Vibrio species.
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