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Related Concept Videos

Upstream Processing01:27

Upstream Processing

97
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Related Experiment Video

Updated: May 5, 2026

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells MSCs and Endothelial Colony Forming Progenitor Cells ECFCs
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Upstream Process Protocol for MSCs Isolated from Different Human-Based Tissue Origins.

Pelin Kılıç1,2, Cansu Özdemir3,4, Begüm Coşar5,6

  • 1Department of Stem Cells and Regenerative Medicine, Stem Cell Institute, Ankara University, Ankara, Türkiye. pkilic@ankara.edu.tr.

Methods in Molecular Biology (Clifton, N.J.)
|July 5, 2024
PubMed
Summary

Mesenchymal stromal/stem cell (MSC) production is crucial for regenerative medicine. This study details an optimized lab-scale protocol for MSC production, supporting advanced therapy medicinal products (ATMPs).

Keywords:
Advanced therapy medicinal products (ATMPs)Bone marrow-derived mesenchymal stromal/stem cells (BM-MSCs)Cell cultureCryopreservationGood Manufacturing Practices (GMP)IsolationMaster cell bank (MCB)PassagingPerinatal tissue-derived mesenchymal stromal/stem cells (PNT-MSCs)Working cell bank (WCB)

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Area of Science:

  • Cell biology
  • Regenerative medicine
  • Biotechnology

Background:

  • Mesenchymal stromal/stem cells (MSCs) are increasingly significant in regenerative medicine and cellular therapeutics.
  • Growing interest in MSCs for diverse medical applications, including advanced therapy medicinal products (ATMPs).
  • Advancements in cell culture technologies enable large-scale MSC production under Good Manufacturing Practices (GMP), ensuring safety and efficacy.

Purpose of the Study:

  • To introduce the increasing significance of MSC production.
  • To outline the growing interest in MSCs for various medical applications.
  • To describe an optimized upstream protocol for laboratory-scale MSC production.

Main Methods:

  • Development of an optimized upstream protocol for laboratory-scale MSC production.
  • Utilizing different tissue sources for MSC isolation.
  • Conducting cell culture in flasks with control of critical parameters.
  • Laying the foundation for downstream processing for ATMP generation.

Main Results:

  • An optimized laboratory-scale protocol for MSC production was described.
  • The protocol controls critical parameters for consistent MSC generation.
  • The described method provides a foundation for downstream processing to generate ATMPs.

Conclusions:

  • MSC production holds significant potential for clinical applications in regenerative medicine.
  • Tailored production processes are essential for generating safe and effective ATMPs.
  • The optimized protocol facilitates laboratory-scale MSC production, supporting future therapeutic development.