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Updated: Jun 21, 2025

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Scalable lipid droplet microarray fabrication, validation, and screening.

Tracey N Bell¹, Aubrey E Kusi-Appiah¹, Vincent Tocci¹

¹Department of Biological Science and Integrative NanoScience Institute, Florida State University, Tallahassee, Florida, United States of America.

|July 5, 2024

Summary

This summary is machine-generated.

Lipid droplet microarrays enable scalable, miniaturized drug screening by delivering lipophilic compounds to cells. This method is adaptable for various cell types and applicable in space-limited or cell-limited settings.

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Last Updated: Jun 21, 2025

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Area of Science:

Biotechnology
Drug Discovery
Cell Biology

Background:

High-throughput screening is essential but costly, limited by space and reagent requirements.
Microarrays are effective for some assays but not widely adopted for cell-based drug discovery due to lipophilic compound delivery challenges.
Lipid droplet microarrays offer a solution for delivering lipophilic drugs to cells in a miniaturized format.

Purpose of the Study:

To scale up lipid droplet microarray fabrication, assay validation, and drug screening processes.
To adapt nanointaglio printing for scalable array production and stabilize arrays for aqueous environments.
To demonstrate the utility of lipid droplet microarrays for delivering both lipophilic and water-soluble compounds to various cell types.

Main Methods:

Adapted nanointaglio printing with a printing press for scalable microarray fabrication.
Utilized a vapor coating process to stabilize lipid droplet arrays for aqueous solution immersion.
Functionalized arrays with extracellular matrix proteins and demonstrated compound delivery to three cell types.

Main Results:

Successfully scaled up lipid droplet array fabrication and stabilization techniques.
Demonstrated successful delivery of both lipophilic and water-soluble compounds to cells.
Screened 92 natural product extracts on a 0.1 cm2 microarray, showing broad applicability.

Conclusions:

Lipid droplet microarrays provide a scalable and versatile platform for miniaturized cell-based drug screening.
This technology is suitable for applications in high biosafety level facilities, space-limited environments, and precision medicine where cell numbers are limited.