Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

p21ras. Heterogeneous localization in transformed cells.

S E Myrdal, N Auersperg

    Experimental Cell Research
    |August 1, 1985
    PubMed
    Summary

    The cellular distribution of p21ras, a protein linked to Kirsten murine sarcoma virus (KiMSV) transformation, is heterogeneous in transformed cells. This specific p21ras localization correlates with cell morphology changes and is reversible.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inhibition of p53 represses E-cadherin expression by increasing DNA methyltransferase-1 and promoter methylation in serous borderline ovarian tumor cells.

    Oncogene·2011
    Same author

    Culture and characterization of human ovarian surface epithelium.

    Methods in molecular medicine·2011
    Same author

    Inhibition of p53 induces invasion of serous borderline ovarian tumor cells by accentuating PI3K/Akt-mediated suppression of E-cadherin.

    Oncogene·2010
    Same author

    The Cytology Program in British Columbia: I. An Evaluation of the Effectiveness of Cytology in the Diagnosis of Cancer and its Application to the Detection of Carcinoma of the Cervix.

    Canadian Medical Association journal·2010
    Same author

    Serous borderline ovarian tumors in long-term culture: phenotypic and genotypic distinction from invasive ovarian carcinomas.

    International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2008
    Same author

    Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro.

    Oncogene·2007

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Virology

    Background:

    • The cellular targets and precise subcellular localization of the Kirsten murine sarcoma virus (KiMSV)-transforming protein, p21ras, remain largely unknown.
    • Previous studies suggested a diffuse distribution of mature p21 on the cytoplasmic face of the plasma membrane.

    Purpose of the Study:

    • To investigate the subcellular distribution of p21ras in normal rat kidney (NRK) cells transformed by KiMSV.
    • To determine if p21ras localization is associated with cellular transformation and morphology.
    • To explore factors influencing p21ras distribution, such as gene overexpression, phosphorylation, and the viral oncogene.

    Main Methods:

    • Indirect immunofluorescent staining of sparse cell cultures after fixation without buffer washes.
    • Analysis of various NRK cell lines: KiMSV-transformed, newly transformed, temperature-sensitive (ts) KiMSV-transformed, and spontaneously transformed.
    • Phase contrast microscopy and scanning electron microscopy (SEM) to observe cellular morphology and ruffling.

    Main Results:

    • A strikingly heterogeneous subcellular distribution of p21ras was observed in transformed NRK cells, contrasting with untransformed cells.
    • This transformation-associated p21ras distribution was independent of gene overexpression, protein phosphorylation, or the viral oncogene.
    • In temperature-sensitive cells, the heterogeneous p21ras distribution was reversible and correlated with acquired transformed morphology, particularly with cellular ruffles and extensions.

    Conclusions:

    • The subcellular localization of p21ras is dynamically regulated and closely linked to the acquisition of a transformed cellular phenotype.
    • The preferential accumulation of p21ras in areas of cellular ruffling suggests a functional role in mediating transformation-induced morphological changes.
    • p21ras's specific localization, rather than mere presence or overexpression, appears critical for its function as a transforming molecule.

    Related Experiment Videos