Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Analysis of Gut Microbiome Dynamics and Dietary Shifts in Three Pollinator Species During Alfalfa Pollination: Insights from Environmental DNA Metabarcoding.

Insects·2026
Same author

Profiling Protein Aggregate Size Using Single-Molecule Array Technology.

Analytical chemistry·2026
Same author

The impact of plant restoration on soil aggregates in karst mountainous areas of southwestern China.

iScience·2026
Same author

Fermented feed-rumen microbiota axis mediates goat kid growth via microbial amino acid synthesis and ruminal epithelial energy metabolism.

NPJ biofilms and microbiomes·2026
Same author

Engineering the <i>Bacillus</i> Transcription Factor CcpC to Construct Citrate-Responsive Biosensors in <i>Escherichia coli</i>.

ACS synthetic biology·2026
Same author

Efficacy and safety of radiofrequency ablation combined with high ligation vs simple radiofrequency ablation for lower extremity varicose veins: a retrospective comparative study.

Wideochirurgia i inne techniki maloinwazyjne = Videosurgery and other miniinvasive techniques·2026

Related Experiment Video

Updated: Jun 21, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

14.5K

Investigating and Engineering an 1,2-Propanediol-Responsive Transcription Factor-Based Biosensor.

Yuxi Teng1, Xinyu Gong1, Jianli Zhang1

  • 1School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.

ACS Synthetic Biology
|July 5, 2024
PubMed
Summary

Researchers engineered a 1,2-propanediol (1,2-PD) biosensor by modifying the PocR transcription factor. This enhanced biosensor offers improved dynamic and operational ranges, expanding its utility in metabolic engineering and bioproduction.

Keywords:
1,2-propanediolPocRRNA polymerase recruiterbifunctional regulationbiosensorshort chain-alcohols

More Related Videos

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

1.4K
Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors
10:44

Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors

Published on: January 31, 2025

564

Related Experiment Videos

Last Updated: Jun 21, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

14.5K
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

1.4K
Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors
10:44

Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors

Published on: January 31, 2025

564

Area of Science:

  • Metabolic Engineering
  • Synthetic Biology
  • Molecular Biology

Background:

  • Transcription factor (TF)-based biosensors are crucial for metabolic engineering but limited in variety.
  • A need exists for novel biosensors to meet diverse bioproduction targets.

Purpose of the Study:

  • To engineer the 1,2-propanediol (1,2-PD)-responsive transcription activator PocR from *Salmonella typhimurium* to expand the biosensor repertoire.
  • To enhance the dynamic and operational ranges of PocR for improved performance in *E. coli*.

Main Methods:

  • Heterologous characterization and promiscuity testing of PocR in *E. coli*.
  • Protein structure prediction (AlphaFold) and mutagenesis to explore PocR's mechanism.
  • Engineering of PocR variants (PocR-F46R/G105D, PocR-ARE) and promoter engineering to create a bifunctional regulator.

Main Results:

  • Initial PocR characterization showed limited operational and dynamic ranges due to promoter binding.
  • Engineered variants demonstrated altered inducer specificity (glycerol) and significantly improved dynamic (6.7-fold) and operational (0-20 mM 1,2-PD) ranges.
  • Successful conversion of PocR to a repressor and development of a 1,2-PD-induced bifunctional regulatory system.

Conclusions:

  • The study successfully engineered a 1,2-PD-responsive transcriptional activator, PocR, significantly enhancing its performance.
  • Developed variants offer a glycerol-responsive activator and a versatile 1,2-PD-induced bifunctional regulator, expanding the biosensor toolbox.
  • This work highlights the potential of exploring and exploiting underexplored transcriptional activators for biotechnological applications.