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Updated: Jun 2, 2026
Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
Published on: April 13, 2022
Regenerative endodontic therapy: From laboratory bench to clinical practice
Xin Shi1, Xiaohan Hu2, Nan Jiang3
1Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China.
Regenerative endodontic therapy offers a new approach to saving teeth with irreversible pulpitis. This review explores tissue engineering strategies, clinical applications, and challenges for pulp-dentin regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Dental pulp vitality is essential for tooth health.
- Irreversible pulpitis and necrosis necessitate advanced treatments.
- Regenerative endodontic therapy (RET) is an emerging alternative to conventional root canal treatment.
Purpose of the Study:
- To review laboratory and clinical issues in pulp-dentin complex regeneration.
- To advance the clinical translation of regenerative endodontic therapy.
- To enhance the quality of life through improved dental treatments.
Main Methods:
- Review of emerging tissue engineering strategies (cell transplantation, cell homing).
- Analysis of current clinical pulp regeneration approaches (direct pulp capping, root revascularization).
- Discussion of biomimetic perspectives (exosomes, cell aggregates) and challenges (infection, inflammation).
Main Results:
- Emerging strategies involve stem cells, biomaterials, and microenvironments.
- Clinical approaches face hurdles but show promise.
- Biomimetic tools like exosomes offer new avenues for translation.
- Addressing infection and inflammation is critical for successful outcomes.
Conclusions:
- Regenerative endodontics requires careful consideration of basic research and clinical application.
- Developing antibacterial and immunomodulatory microenvironments is key.
- Overcoming challenges will enhance the efficacy and translation of RET protocols.
