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Updated: Jun 21, 2025
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
Published on: January 2, 2018
A Model of Linear Response with Progressive Decay Equally Suited for RiboGreen® Quantitation of RNA and Dissolution
Lee J Klein1, Susannah Gaye1, Morgan Crawford2
1Merck & Co., Inc., 770 Sumneytown Pike, West Point, PA 19486 USA.
Abstract:
A new regression model is presented which offers flexibility, freedom from subjective determinations of linear range, and very wide applicability to measurement systems of industrial importance. This "progressive decay" model starts as a deceptively simple ordinary differential equation. We show here that its solution faithfully describes real but seemingly unconnected data from a plate-based assay for quantitation of RNA with RiboGreen® and dissolution data for a triple fixed-dose combination solid oral dosage form.
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