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Updated: Jun 21, 2025

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
Published on: May 27, 2021
Studying lipid flip-flop in asymmetric liposomes using 1H NMR and TR-SANS
Dominik Dziura1, Maksymilian Dziura1, Drew Marquardt2
1Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada.
Abstract:
The specific spatial and temporal distribution of lipids in membranes play a crucial role in determining the biochemical and biophysical properties of the system. In nature, the asymmetric distribution of lipids is a dynamic process with ATP-dependent lipid transporters maintaining asymmetry, and passive transbilayer diffusion, that is, flip-flop, counteracting it. In this chapter, two probe-free techniques, 1H NMR and time-resolved small angle neutron scattering, are described in detail as methods of investigating lipid flip-flop rates in synthetic liposomes that have been generated with an asymmetric bilayer composition.
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