Automated high-performance immunosorbent assay for recombinant leukocyte A interferon

Area of Science:

• Biochemistry
• Immunology
• Analytical Chemistry

Background:

• Recombinant leukocyte A interferon is a crucial therapeutic agent.
• Accurate and efficient quantification methods are essential for its production and quality control.
• Existing methods like enzyme immunoassay can be time-consuming.

Purpose of the Study:

• To develop a rapid and automated assay for quantifying recombinant leukocyte A interferon.
• To compare the efficiency and simplicity of the new assay with existing methods.

Main Methods:

• Development of a small immunosorbent column using monoclonal antibody immobilized on silica.
• Automation of the assay procedure for sample injection and microprocessor-controlled analysis.
• Validation of the assay's performance, including analysis time, concentration range, and reproducibility.

Main Results:

• The automated assay provides results in under 20 minutes.
• The assay operates effectively in the concentration range of 0.02-0.3 mg/ml.
• High reproducibility was demonstrated with a standard deviation of 3.96% and a correlation coefficient of 0.9996.

Conclusions:

• The developed automated immunosorbent assay is a rapid and simple method for recombinant leukocyte A interferon quantification.
• This technique offers significant advantages in terms of speed and ease of use compared to enzyme immunoassays.