Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Misuse and interlaboratory test reproducibility of API 20E system.

B Holmes, C A Dawson

    Journal of Clinical Pathology
    |August 1, 1985
    PubMed
    Summary

    Many bacterial strains referred for identification could not be identified using the API 20E system due to user error or technical issues. Proper methodology is crucial for accurate microbial identification with the API 20E system.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Retraction Note: Therapeutic potential of targeting IRES-dependent c-myc translation in multiple myeloma cells during ER stress.

    Oncogene·2023
    Same author

    A technique for retrieving misplaced intramedullary metalwork during total knee replacement.

    Annals of the Royal College of Surgeons of England·2019
    Same author

    mTORC2/AKT/HSF1/HuR constitute a feed-forward loop regulating Rictor expression and tumor growth in glioblastoma.

    Oncogene·2017
    Same author

    Therapeutic potential of targeting IRES-dependent c-myc translation in multiple myeloma cells during ER stress.

    Oncogene·2015
    Same author

    Microbial water quality communication: public and practitioner insights from British Columbia, Canada.

    Journal of water and health·2014
    Same author

    An exploration of spatial risk assessment for soil protection: estimating risk and establishing priority areas for soil protection.

    The Science of the total environment·2014

    Area of Science:

    • Microbiology
    • Clinical Diagnostics

    Background:

    • The API 20E system is a common method for bacterial identification.
    • Accurate identification is essential for patient care and epidemiological studies.

    Purpose of the Study:

    • To evaluate the reasons for bacterial identification failures using the API 20E system.
    • To assess the performance of the API 20E system in a real-world diagnostic setting.

    Main Methods:

    • One hundred bacterial strains were analyzed.
    • Identification was attempted using the API 20E system.
    • Results were compared with sender's data and conventional methods when possible.

    Main Results:

    • 31% of strains were unidentifiable due to procedural errors.
    • 26% were correctly identified by the sender.
    • 18% of previously unidentified strains were identified by the API 20E system in this study.
    • An average interlaboratory error rate of 6.1% was observed for API 20E tests.

    Conclusions:

    • User error is a significant factor in API 20E identification failures.
    • The API 20E system can successfully identify a portion of strains that were initially unidentifiable.
    • Standardization of protocols is recommended to improve the reliability of bacterial identification.

    Related Experiment Videos