Related Experiment Video
Updated: Jun 21, 2025
07:55
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Published on: September 11, 2022
1.8K
RAGging on recombination signal sequence strength for diffusion-mediated recombination.
1Immunology Program, Garvan Institute of Medical Research, Darlinghurst, New South Wales, Australia.
Immunology and Cell Biology
|July 8, 2024
Summary
Recombination signal sequences (RSS) in the IGKV locus are more efficient at mediating V(D)J recombination than those in the IGH locus. This difference is likely due to distinct molecular mechanisms driving recombination activating gene (RAG) activity.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- V(D)J recombination is a crucial process for adaptive immune receptor gene assembly.
- Immunoglobulin (IG) loci, including IGKV and IGH, undergo V(D)J recombination.
- Recombination signal sequences (RSS) are critical elements guiding this process.
Purpose of the Study:
- To explore the distinct mechanisms underlying V(D)J recombination at different immunoglobulin loci.
- To investigate why RSS in the IGKV locus are more efficient mediators of recombination activating gene (RAG) recombination than those in the IGH locus.
Main Methods:
- Comparative analysis of RSS function between IGKV and IGH loci.
- Investigating the molecular mechanisms of RAG-mediated recombination.
Main Results:
- RSS within the IGKV locus demonstrate enhanced efficiency in mediating V(D)J recombination compared to the IGH locus.
- This difference in RSS strength is proposed to stem from variations in the molecular mechanisms governing RAG-mediated recombination between these loci.
Conclusions:
- Distinct molecular mechanisms drive V(D)J recombination efficiency at different immunoglobulin loci.
- Understanding these locus-specific mechanisms provides new insights into immune receptor gene diversification.
Related Concept Videos
02:53Conservative Site-specific Recombination and Phase Variation
6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.0K
02:31Homologous Recombination
50.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.4K
01:30Crossing Over
4.3K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
4.3K
02:08Gene Conversion
9.7K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.7K
02:32Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K