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Updated: Jun 21, 2025
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Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
Published on: August 25, 2019
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Preimplantation Genetic Testing for Aneuploidy in In Vitro Fertilization Using Comprehensive Chromosome Screening: A
Omur Taskin1, Alyssa Hochberg2, Justin Tan1
1Department of Obstetrics and Gynaecology, University of British Columbia, Children's and Women's Hospital and Health Centre of British Columbia, Vancouver, Canada.
International Journal of Fertility & Sterility
|July 8, 2024
Summary
Pre-implantation genetic testing (PGT-A) with comprehensive chromosome screening (CCS) did not improve pregnancy rates in randomized controlled trials (RCTs). While miscarriage rates decreased in the overall group, PGT-A
Area of Science:
- Reproductive Medicine
- Genetics
- Clinical Trials
Background:
- The clinical utility of pre-implantation genetic testing for aneuploidy (PGT-A) remains debated, with conflicting evidence from meta-analyses and recent randomized controlled trials (RCTs).
- Comprehensive chromosome screening (CCS) is a common method used in PGT-A to assess embryo ploidy.
- Understanding the impact of PGT-A on in vitro fertilization (IVF) outcomes is crucial for clinical decision-making.
Purpose of the Study:
- To evaluate the benefits of PGT-A using CCS on IVF outcomes.
- To analyze effects on clinical pregnancy rate (CPR), ongoing pregnancy rate (OPR), and miscarriage rate (MR) among RCTs.
Main Methods:
- Systematic search for RCTs comparing PGT-A with CCS versus no PGT-A from inception to December 2020.
- Random effects meta-analysis to calculate odds ratios (OR) for CPR, OPR, and MR.
- Assessment of heterogeneity using Forest plots and I2 statistics; publication bias evaluated using Egger's test.
Main Results:
- Meta-analysis of seven RCTs (1251 citations) found no significant improvement in OPRs or CPRs with PGT-A across all age groups.
- A decrease in MR was observed with PGT-A in the overall group and for blastocyst biopsies, but not when stratified by age (<35 and ≥35 years).
- Embryo biopsies were performed at various stages: polar body, day 3, and day 5-6.
Conclusions:
- Current evidence from RCTs does not support a significant benefit of PGT-A with CCS in improving pregnancy rates.
- Further research with standardized protocols is needed to definitively determine the clinical value and optimal application of PGT-A.
- More data are required to fully understand the risks and advantages of PGT-A for patient counseling and practice.
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