Effect of hyperbilirubinemia and phototherapy on apoptotic microparticle levels in neonates

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Updated: Jul 25, 2026

Biochemical Measurement of Neonatal Hypoxia
13:13

Biochemical Measurement of Neonatal Hypoxia

Published on: August 24, 2011

14.1K

Ebru Yücesoy1, Fatma Demir Yenigürbüz2, Halil Ateş3

  • 1Department of Pediatrics, Division of Neonatology.

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|July 8, 2024
PubMed
Abstract

Insights

Neonates with nonhemolytic pathologic hyperbilirubinemia have increased apoptotic microparticles (MPs), particularly endothelial-derived ones. Phototherapy did not significantly alter these apoptotic MP levels in the study group.

Area of Science:

  • Neonatal physiology
  • Hematology
  • Cell biology

Background:

  • Hyperbilirubinemia is common in neonates.
  • Pathologic hyperbilirubinemia can have serious consequences.
  • Microparticles (MPs) are circulating vesicles released from cell membranes.

Purpose of the Study:

  • To investigate the impact of nonhemolytic pathologic hyperbilirubinemia and phototherapy on various types of apoptotic microparticles (MPs) in neonates.
  • To compare apoptotic MP levels in neonates with and without hyperbilirubinemia.
  • To assess changes in apoptotic MP levels before and after phototherapy.

Main Methods:

  • Flow cytometry was used to analyze peripheral blood samples from 33 neonates with nonhemolytic pathologic hyperbilirubinemia and 25 healthy controls.
  • Apoptotic MPs were identified as Annexin V-positive.
  • Platelet-derived MPs (CD31+), endothelial-derived MPs (CD144+), and TF-positive MPs (CD142+) were quantified.

Main Results:

  • Total apoptotic and endothelial-derived apoptotic MPs were significantly higher in neonates with hyperbilirubinemia compared to controls, both before and after phototherapy.
  • No significant differences were observed in total apoptotic, platelet-derived, endothelial-derived, or TF-positive apoptotic MPs before and after phototherapy within the hyperbilirubinemia group.

Conclusions:

  • Nonhemolytic pathologic hyperbilirubinemia is associated with elevated levels of total and endothelial-derived apoptotic MPs.
  • Phototherapy does not appear to significantly affect these apoptotic MP levels.
  • Further research is warranted to explore the potential link between elevated apoptotic MPs and thromboembolism risk in neonates.

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