Related Experiment Video
Updated: Sep 7, 2026

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
Published on: June 14, 2016
Estimation of protein molecular weights by high performance molecular-sieve chromatography on agarose columns in
Abstract:
Calibration curves showing the linear relationship between -log KD and (molecular weight)2/3 are presented for proteins subjected to molecular-sieve chromatography in a 0.1% sodium dodecyl sulphate solution on crosslinked gels of different agarose concentrations (5, 9, 12 and 20%). These gels permit separation and estimation of molecular weights of proteins in the range 5000 to about 400 000, 130 000, 130 000, and 100 000, respectively. Separations of model proteins, heavy and light chains of gamma-globulin, and cyanogen bromide fragments of cellulase are shown.
More Related Videos
10:41Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
10:00Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Published on: June 20, 2019
Related Concept Videos
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...