Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

1.6K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
1.6K
IR Spectrometers01:25

IR Spectrometers

1.1K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.1K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.7K
IR Spectrum01:19

IR Spectrum

977
When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
977
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

3.0K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
3.0K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.3K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optically Tunable Many-Body Exciton-Phonon Quantum Interference.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024
Same author

The Edifice of Vasculature-On-Chips: A Focused Review on the Key Elements and Assembly of Angiogenesis Models.

ACS biomaterials science & engineering·2024
Same author

Functionalization of <sup>68</sup>Ga-Radiolabeled Nanodiamonds with Octreotide Does Not Improve Tumor-Targeting Capabilities.

Pharmaceuticals (Basel, Switzerland)·2024
Same author

Enhanced Vascular-like Network Formation of Encapsulated HUVECs and ADSCs Coculture in Growth Factors Conjugated GelMA Hydrogels.

ACS biomaterials science & engineering·2024
Same author

The Effects of Negative Pressure Therapy on Hair Growth of Mouse Models.

Tissue engineering. Part A·2024
Same author

3D-Bioprinted GelMA Scaffold with ASCs and HUVECs for Engineering Vascularized Adipose Tissue.

ACS applied bio materials·2023

Related Experiment Video

Updated: Jun 21, 2025

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

1.2K

Cytometry in the Short-Wave Infrared.

Te-I Liu1, Jhih-Shan Wang1,2,3, Ai-Phuong Nguyen1,4

  • 1Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei City 106319, Taiwan.

ACS Nano
|July 8, 2024
PubMed
Summary

We developed short-wave infrared (SWIR) flow and image cytometry to overcome conventional limitations. These methods enable sensitive detection of SWIR fluorophores in cells, revealing new biological insights.

Keywords:
NIR-II windowantioxidantflow cytometryimage cytometryreactive oxygen speciessingle-wall carbon nanotubes

More Related Videos

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.2K
Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
04:07

Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments

Published on: February 23, 2024

1.2K

Related Experiment Videos

Last Updated: Jun 21, 2025

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

1.2K
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.2K
Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
04:07

Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments

Published on: February 23, 2024

1.2K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Spectroscopy

Background:

  • Conventional cytometry is limited by a restricted optical window (400-850 nm), hindering simultaneous detection of multiple fluorophores.
  • This limitation impedes the study and application of short-wave infrared (SWIR) fluorophores in cellular research.

Purpose of the Study:

  • To introduce novel SWIR-based cytometry methods for enhanced cellular analysis.
  • To develop a protocol for quantifying cellular fluorophore mass.
  • To address spectral overlapping issues in traditional cytometry.

Main Methods:

  • Development of SWIR flow cytometry and SWIR image cytometry systems.
  • Establishment of a quantification protocol for deducing cellular fluorophore mass.
  • Utilized individualized, high-purity (6,5) single-wall carbon nanotubes as model fluorophores in RAW264.7 macrophage-like cells.

Main Results:

  • Achieved a high sensitivity limit of detection of approximately 0.1 fg cell⁻¹ within a 30-minute timeframe.
  • Demonstrated that low-dose (6,5) single-wall carbon nanotubes exhibit antioxidant properties.
  • Observed a dose-dependent correlation between (6,5) uptake, cell morphology, and oxidative stress.

Conclusions:

  • SWIR cytometry offers a sensitive and effective solution for analyzing SWIR fluorophores in cells.
  • The developed methods overcome spectral overlapping limitations inherent in conventional cytometry.
  • Provides a powerful tool for advancing cell biology research and applications utilizing SWIR fluorophores.