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No evidence on infectious DNA-based agents in pediatric acute lymphoblastic leukemia using whole metagenome shotgun

Amadeus T Heinz1,2, Silke Grumaz3, Christoph Slavetinsky4

  • 1Department of Pediatric Hematology and Oncology, University Children´s Hospital Tuebingen, Tuebingen, Germany.

Frontiers in Cellular and Infection Microbiology
|July 8, 2024
PubMed

Insights

The cause of pediatric acute lymphatic leukemia (ALL) remains unknown. This study found no significant bacterial or viral DNA in bone marrow of children with ALL, suggesting these microbes are not a direct cause.

Area of Science:

  • Pediatric Oncology
  • Microbiology
  • Genomics

Background:

  • The etiology of pediatric acute lymphatic leukemia (ALL) is not fully understood.
  • Investigating potential microbial causes is crucial for understanding ALL development.

Purpose of the Study:

  • To investigate the presence of bacterial and viral DNA in bone marrow of children with treatment-naïve ALL.
  • To explore potential microbial etiological factors in pediatric ALL.

Main Methods:

  • Whole-metagenome shotgun sequencing was performed on bone marrow samples from children with ALL (n=6) and controls (n=6).
  • Peripheral blood samples were also analyzed.
  • Bioinformatic analysis was used to identify and quantify microbial DNA, with rigorous contaminant control.

Main Results:

  • No significant amounts of bacterial or viral DNA were detected in the bone marrow samples of children with ALL after bioinformatic analysis.
  • Control samples also showed no significant microbial DNA presence.

Conclusions:

  • This study did not identify a significant role for bacterial or viral DNA in the bone marrow of treatment-naïve pediatric ALL patients.
  • The findings suggest that common microbial agents are unlikely to be a direct cause of pediatric ALL.
Keywords:
ALLhigh-throughput sequencinginfectious etiologymicrobiomeshotgun sequencing

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