A multidot immunobinding assay for autoimmune testing: evaluation for the diagnosis and management of connective...

Multidot immunobinding assays were evaluated for applicability to connective tissue diseases. Four groups of human sera [(a) healthy, (b) miscellaneous diseases, (c) rheumatoid arthritis and (d) other connective tissue diseases] were used to obtain profiles comprising antibodies against DNA, various subcellular components and rheumatoid factor (RF). Qualitative correlation with standard tests for antinuclear factor and RF was shown. Statistically significant ranges of titer for groups (c) and (d) were obtained, and monitoring individual patients during the progression of disease was demonstrated.