Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
Transcription Initiation01:47

Transcription Initiation

16.4K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.4K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

24.1K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.1K
Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

1.8K
1.8K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

1.6K
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative performance evaluation of machine learning classifiers for multi-class intrusion detection on the NSL-KDD dataset.

Scientific reports·2026
Same author

Arterial switch operation after left ventricular retraining with ductal stent: Our experience.

Annals of pediatric cardiology·2026
Same author

Polystyrene Microplastics Activate Noncanonical TGF‑β Signaling and Metabolomic Reprogramming to Promote Epithelial-Mesenchymal Transition and Fibrosis in the Kidney.

ACS omega·2026
Same author

Identification and Computational validation of ferroptosis suppressor genes as therapeutic targets in pancreatic cancer.

Discover oncology·2026
Same author

Educational Background and Demographic Characteristics of Anesthesiology Department Chairs in the United States.

Anesthesiology open·2026
Same author

Synthesis and multi-target biological evaluation of Schiff base metal complexes: tuning anticancer efficacy and enzyme inhibition via metal center variation.

Bioorganic chemistry·2026

Related Experiment Video

Updated: Jun 21, 2025

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.7K

MediatorWeb: a protein-protein interaction network database for the RNA polymerase II Mediator complex.

Sourobh Maji1,2,3, Mohd Waseem4,5, Manish Kumar Sharma4

  • 1Plant Transcription Regulation, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.

The FEBS Journal
|July 8, 2024
PubMed
Summary

MediatorWeb is a new database for analyzing the protein-protein interaction (PPI) network of the Mediator complex. It aids in understanding Mediator subunits

Keywords:
Mediator complexMediator subunitnetworkprotein–protein interactiontranscription

More Related Videos

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

8.1K
High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

14.2K

Related Experiment Videos

Last Updated: Jun 21, 2025

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.7K
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

8.1K
High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

14.2K

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Systems Biology

Background:

  • The Mediator complex is crucial for transcription regulation, with its protein-protein interaction (PPI) network being highly dynamic.
  • Understanding the Mediator complex's structure and function across different species is essential for deciphering gene expression regulation.

Purpose of the Study:

  • To introduce MediatorWeb, an interactive web-based platform for comparative analysis of Mediator complex subunits.
  • To facilitate visualization and analysis of Mediator subunit PPI networks across humans, yeast, and plants.

Main Methods:

  • Development of a user-friendly web interface database, MediatorWeb.
  • Comparative analysis of Mediator subunits from humans, Saccharomyces cerevisiae, and Arabidopsis thaliana.
  • Network visualization of the Mediator complex and its interacting proteins, including functional annotations.
  • Access to secondary/tertiary structures and residue-level contact information.
  • Interolog detection based on orthologous analyses for cross-species functional inference.

Main Results:

  • MediatorWeb provides downloadable PPI network data for the Mediator complex, submodules, and individual subunits.
  • Functional annotations linked to interacting proteins offer insights into Mediator subunit roles.
  • Structural and residue-level information is readily accessible for comparative studies.
  • Interolog analysis enables functional predictions in understudied organisms.

Conclusions:

  • MediatorWeb serves as a valuable resource for researchers studying the Mediator complex's role in transcription regulation.
  • The platform enhances the understanding of Mediator subunit functions and their evolutionary conservation.
  • Comparative analysis facilitated by MediatorWeb can uncover novel insights into gene expression mechanisms.