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Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
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Generating Neuroimmune Assembloids Using Human Induced Pluripotent Stem Cell (iPSC)-Derived Cortical Organoids and
Kriti Kalpana1, Chandrika Rao1, Stefan Semrau1
1The New York Stem Cell Foundation Research Institute, New York, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 8, 2024
Summary
Researchers developed a new method to create brain organoids with microglia, the brain's immune cells. This neuroimmune assembloid model aids in studying neurological diseases and cell interactions.
Area of Science:
- Neuroscience
- Immunology
- Stem Cell Biology
Background:
- Brain organoids model human neurodevelopment and disease.
- Microglia, the CNS immune cells, are crucial in neurological disorders but often excluded from organoid models.
Purpose of the Study:
- To develop a method for generating neuroimmune assembloids integrating human induced pluripotent stem cell (iPSC)-derived cortical organoids and microglia.
- To provide a comprehensive protocol for studying neuro-immune interactions in vitro.
Main Methods:
- Generation of iPSC-derived cortical organoids.
- Two methods for microglia integration: direct addition or co-aggregation with neuronal progenitors.
- Protocols for single-cell RNA sequencing, fixation, cryosectioning, and immunostaining.
Main Results:
- Successful long-term survival and maturation of microglia within organoids.
- Demonstration of two viable methods for creating neuroimmune assembloids.
- Establishment of protocols for comprehensive downstream analysis.
Conclusions:
- The developed protocol enables the creation of advanced neuroimmune assembloids.
- This model system is valuable for investigating microglia roles in neurological diseases.
- Facilitates research on neuro-immune cell interactions in vitro.
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