Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

1.2K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A F<sub>420</sub>-dependent Single Domain Chemogenetic Tool for Protein De-dimerization.

Journal of molecular biology·2025
Same author

Automated Plate Reader-Based Assays of Light-Activated GPCRs.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Modular Light-Emitting Diode Shelving Systems for Scalable Optogenetics.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors.

Nature communications·2023
Same author

CDK6 activity in a recurring convergent kinase network motif.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2023
Same author

Structure-guided optimization of light-activated chimeric G-protein-coupled receptors.

Structure (London, England : 1993)·2022
Same journal

Females Are Completely Resistant to Semaglutide-Induced Muscle Loss in ob/ob Mice.

Diabetes·2026
Same journal

Ketone Bodies Derived From Medium-Chain Triglycerides Support Brain Metabolism and Function Under Hypoglycemia in Type 1 Diabetes Mellitus.

Diabetes·2026
Same journal

Targeting the ADA/ADO Axis Rescues β-Cell Failure in Type 2 Diabetes.

Diabetes·2026
Same journal

TXNIP Is Positioned as a Key Mediator of Hyperglycemia-Induced Vascular Senescence.

Diabetes·2026
Same journal

Sex Differences in ER Stress Pathways Are a Key Determinant of β-Cell Proliferation and Resilience.

Diabetes·2026
Same journal

The Mechanism of TNF-α Combined With High Glucose in Regulating Calnexin Aggravates Endoplasmic Reticulum Stress in Endothelial Cell Injury of Diabetic Retinopathy.

Diabetes·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2025

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

1.9K

Optogenetics in Pancreatic Islets: Actuators and Effects.

Christina G Gangemi1,2,3, Harald Janovjak1

  • 1Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Bedford Park, South Australia, Australia.

Diabetes
|July 8, 2024
PubMed
Summary
This summary is machine-generated.

Optogenetics offers precise control over pancreatic islet cells, advancing our understanding of hormone secretion and glucose homeostasis. This technology is crucial for exploring diabetes mellitus mechanisms and potential therapies.

More Related Videos

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
11:24

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Published on: May 14, 2019

5.4K
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

65.2K

Related Experiment Videos

Last Updated: Jun 21, 2025

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

1.9K
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
11:24

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Published on: May 14, 2019

5.4K
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

65.2K

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Islets of Langerhans are vital endocrine microorgans in the pancreas, regulating blood glucose through hormone secretion (e.g., insulin, glucagon).
  • Islet cell function involves complex molecular dynamics, and their dysfunction is linked to diabetes mellitus.
  • Optogenetics, using light-sensitive actuators, enables precise manipulation of cellular functions.

Purpose of the Study:

  • To review the application of optogenetics in islet cell biology.
  • To discuss optogenetic actuators and their impact on islet function and diabetes research.
  • To highlight emerging studies on paracrine regulation and intracellular signaling within islets.

Main Methods:

  • Harnessing natural photoreceptor proteins and engineered variants for optogenetic manipulation.
  • Applying optogenetic tools to mouse and human islet cells.
  • Reviewing existing literature on optogenetics in islet studies.

Main Results:

  • Optogenetics allows for spatiotemporal control of islet cell activity, including hormone production and secretion.
  • Emerging research explores optogenetics for studying intercellular communication and signaling pathways in islets.
  • Optogenetic approaches provide new avenues for investigating the pathophysiology of diabetes.

Conclusions:

  • Optogenetics is a powerful tool for dissecting islet cell function and its role in metabolic diseases.
  • Continued development of optogenetic actuators will enhance our understanding of glucose homeostasis.
  • This technology holds promise for future therapeutic strategies for diabetes mellitus.