Related Experiment Video
Updated: Jun 16, 2026
09:07
Generation of Transgenic Rats using a Lentiviral Vector Approach
Published on: May 17, 2020
8.6K
Generation and Characterization of a Novel Prkcd-Cre Rat Model
Sanne Toivainen1, Michele Petrella1, Li Xu1
1Department of Clinical and Experimental Medicine, Linkoping University, Linkoping 58225, Sweden.
Summary
Researchers developed a new rat model to study central amygdala PKCδ+ neurons, crucial for appetite, anxiety, and addiction. This tool enables new investigations into these behaviors.
Area of Science:
- Neuroscience
- Genetics
- Behavioral Science
Background:
- Central amygdala (CeA) PKCδ-expressing neurons influence appetite, anxiety, pain, and addiction.
- Existing research primarily uses mouse models, lacking specific genetic tools for rats.
Purpose of the Study:
- To generate and characterize a novel transgenic Prkcd-cre knock-in rat model.
- To enable selective manipulation of PKCδ+ neurons in rats for behavioral research.
Main Methods:
- CRISPR/Cas9 gene editing to create the Prkcd-cre rat.
- Anatomical, electrophysiological, and behavioral analyses in both sexes.
- Optogenetic stimulation and real-time place preference tasks.
Main Results:
- Successful generation of Prkcd-cre rats with selective Cre expression in CeA PKCδ+ cells.
- Projections of PKCδ+ neurons align with mouse data.
- Optogenetic stimulation of CeA PKCδ+ neurons reduced palatable food intake and induced aversion.
Conclusions:
- The Prkcd-cre rat line is a valuable new tool for studying PKCδ+ neuron function.
- This model complements existing mouse lines for neuroscience research.
- Provides a platform for investigating the role of PKCδ+ neurons in motivated behaviors.
