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Defining blood hematology reference values in female pig-tailed macaques (Macaca nemestrina) using the Isolation
Daniel Kim1, Abigail Derton2, George Khalil3
1Laboratory Branch, Division of HIV Prevention, National Center for HIV, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Journal of Medical Primatology
|July 9, 2024
Summary
This study established normal hematological ranges for pig-tailed macaques (PTMs) using the Isolation Forest algorithm. This reference index aids in detecting drug toxicities in preclinical HIV prevention research.
Area of Science:
- Primate models
- Hematology
- Pharmacology
Background:
- Pig-tailed macaques (PTMs) are crucial preclinical models for evaluating antiretroviral drugs in HIV prevention.
- Hematological changes often signal drug toxicities and disease pathologies in these models.
- Accurate assessment of pharmaceutical safety relies on understanding normal hematological values.
Purpose of the Study:
- To define normal hematological ranges in PTMs for improved pharmaceutical safety assessment.
- To establish a reliable reference index for preclinical drug evaluation studies.
- To utilize advanced algorithms for robust data analysis in non-human primate research.
Main Methods:
- Analyzed complete blood counts from 18 female PTMs (159 samples).
- Employed the Isolation Forest (iForest) algorithm to identify hematological outliers.
- Calculated median, IQR, and ranges for 13 key hematology parameters.
Main Results:
- Identified 22 outliers from the total samples.
- Excluded detected outliers to establish a refined reference index.
- Defined specific normal ranges for hematological parameters in female PTMs.
Conclusions:
- Successfully established a normal hematological reference index for female PTMs using iForest.
- This index serves as a valuable tool for future drug toxicity studies.
- Enhances the reliability of PTMs as preclinical models for HIV prevention research.
